Application of biclustering of gene expression data and gene set enrichment analysis methods to identify potentially disease causing nanomaterials

• Andrew Williams and
• Sabina Halappanavar

Beilstein J. Nanotechnol. 2015, 6, 2438–2448, doi:10.3762/bjnano.6.252

• examined NM-induced pulmonary toxicity. These results are presented in Figure 2. Bicluster-3 (genes associated with chemokine activity reflecting pulmonary inflammation) was enriched for most of the NMs. These results are in alignment with other studies in the literature that have shown pulmonary

Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices

• Stephanie Hirn,
• Nadine Haberl,
• Kateryna Loza,
• Matthias Epple,
• Wolfgang G. Kreyling,
• Barbara Rothen-Rutishauser,
• Markus Rehberg and
• Fritz Krombach

Beilstein J. Nanotechnol. 2014, 5, 2440–2449, doi:10.3762/bjnano.5.253

• value in detecting nanomaterial pulmonary toxicity [47]. Here we show that there was no cytotoxic response of PCLS to micron-sized quartz particles, only a slight cytotoxic response to PVP-coated Ag-NPs, but a strong cytotoxic response to uncoated ZnO-NPs. Moreover, none of the materials induced a

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• silver nanoparticles in vivo induced a moderate pulmonary toxicity, however, only at rather high concentrations. The same was found in precision-cut lung slices of rats in which silver nanoparticles remained mainly at the tissue surface. In a human 3D triple-cell culture model consisting of three cell

• intratracheal instillation can cause moderate pulmonary toxicity in vivo, but only at rather high concentrations [124]. Ex vivo approaches, such as isolated-perfused lungs or precision-cut lung slices (PCLS), have been developed as an alternative to in vivo studies [125]. They allow for a more detailed view on

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

• Nadine Haberl,
• Stephanie Hirn,
• Alexander Wenk,
• Jörg Diendorf,
• Matthias Epple,
• Blair D. Johnston,
• Fritz Krombach,
• Wolfgang G. Kreyling and
• Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

• AgNP can induce moderate pulmonary toxicity, but only at rather high concentrations. Keywords: cytotoxicity; inflammation; pulmonary toxicity; silver nanoparticles; Introduction Silver nanoparticles (AgNP) are among the most promising nanomaterials, and their usage in medical applications and

• products, the lungs are considered to be the main portal of entry for AgNP into the human body [29]. With regard to the lack of knowledge of the in vivo pulmonary toxicity of AgNP, the aim of the current study was to assess the adverse health effects of AgNP after the intratracheal instillation in rats

• µg, of 70 nm monodisperse PVP-coated AgNP caused cytotoxic and inflammatory responses in lungs of healthy, adult female rats, as shown by elevated BALF LDH, protein, and cytokine levels as well as neutrophil numbers. These findings suggest that exposure to inhaled AgNP can induce a moderate pulmonary